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A bioinformatic approach to the identification of a conserved domain in a sugarcane legumain that directs GFP to the lytic vacuole
Jackson, M. A., Rae, A. L., Casu, R. E., Grof, C. P. L., Bonnett, G. D. and Maclean, D. J. (2007) A bioinformatic approach to the identification of a conserved domain in a sugarcane legumain that directs GFP to the lytic vacuole. Functional Plant Biology, 34 : 633-644.
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| Author(s) |
Jackson, M. A.
Rae, A. L.
Casu, R. E.
Grof, C. P. L.
Bonnett, G. D.
Maclean, D. J.
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| Title |
A bioinformatic approach to the identification of a conserved domain in a sugarcane legumain that directs GFP to the lytic vacuole
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| Journal name |
Functional Plant Biology
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| Publication date |
2007
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| Year available |
2007
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| Volume number |
34
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| ISSN |
1445-4408
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| Start page |
633
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| End page |
644
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| Total pages |
12
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| Editor(s) |
Henry, J.
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| Place of publication |
Australia
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| Publisher |
CSIRO Publishing
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| Collection year |
2008
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| Subject |
C1
270402 Plant Physiology
780105 Biological sciences
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| Abstract |
Sugarcane is an ideal candidate as a biofactory for the production of alternate higher value products. One way of achieving this is to direct useful proteins into the vacuoles within the sugarcane storage parenchyma tissue. By bioinformatic analysis of gene sequences from putative sugarcane vacuolar proteins a motif has been identified that displays high conservation across plant legumain homologues that are known to function within vacuolar compartments. This. ve amino acid motif, represented by the sequence IRLPS in sugarcane is shown to direct an otherwise secreted GFP fusion protein into a large acidic and proteolytic vacuole in sugarcane callus cells as well as in diverse plant species. In mature sugarcane transgenic plants, the stability of GFP appeared to be dependent on cell type, suggesting that the vacuolar environment can be hostile to introduced proteins. This targeting motif will be a valuable tool for engineering plants such as sugarcane for production of novel products.
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| Keyword(s) |
Plant Sciences
biofactory
vacuole processing enzyme
Saccharum
vacuole targeting
Arabidopsis-thaliana
Plant-cells
Aspartic Proteinase
Fusion Proteins
Propeptide
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